Immuno-Oncology and Immunotherapy Part D

Immuno-oncology and immunotherapy, Part D, Volume 196 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including Investigating the endothelial-immune axis in cancer, Establishing Tyro3, Axl, and Mertk Chinese Hamster Ovary (CHO) Reporter Cell Lines for Cancer Immunology and Therapeutic Applications, Amino acid analog-based labelling of the immunomodulatory cancer secretome, Human and mouse iNKT cell expansion and engineering with viral vectors, Next-generation deconvolution of the tumor microenvironment with omnideconv, and much more. Additional sections cover Comprehensive prediction of tumor neoantigens with NextNEOpi, Ex vivo profiling of organotypic tumor spheroids in 3D microfluidic culture, A flow cytometry-based assay for evaluation of T cell activation and degranulation in coculture systems, An adenosine production assay suitable for 2D and 3D cancer cell culture, Determination of lactate in the tumor interstitial fluid, Combined in vitro differentiation and cell sorting-based isolation of highly pure mouse bone marrow-derived basophils, Organ-on-Chip immunostaining method for three-dimensional identification and study of immune cells responding to drug-treated tumor cells, and much more.